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loading control protein α tubulin  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc loading control protein α tubulin
    Loading Control Protein α Tubulin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1540 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+loading+control/alpha-Tubulin+Mouse+mAb/pm41862734-96-7-15
    Average 97 stars, based on 1540 article reviews
    loading control protein α tubulin - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Control:

    Article Title: DPP an extracellular matrix molecule induces Wnt5a mediated signaling to promote the differentiation of adult stem cells into odontogenic lineage.
    Article Snippet: .. Actin was used as protein loading control while tubulin and lamin A/C (#4777 Cell signaling) were used for cytoplasmic and nuclear fractions respectively. ..

    Article Title: Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells
    Article Snippet: Protein concentrations were determined using a BCA assay (Pierce), and equal amounts of protein from different samples were loaded onto a 4–15% precast gel (Bio-Rad). .. SOX10 was detected using a mouse anti-SOX10 antibody (1:1000, sc-271163, Santa Cruz Biotechnology). α-Tubulin was used as a protein loading control (1:2000, #2144, Cell Signaling Technology). .. The plasmids generated in this study have been deposited and pending in Addgene.

    Article Title: Antagonistic roles for MITF and TFE3 in melanoma plasticity.
    Article Snippet: Article

    Article Title: Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells
    Article Snippet: Protein concentrations were determined using a BCA assay (Pierce), and equal amounts of protein from different samples were loaded onto a 4%–15% precast gel (Bio-Rad). .. SOX10 was detected using a mouse anti-SOX10 antibody (1:1,000, sc-271163, Santa Cruz Biotechnology). α-Tubulin was used as a protein loading control (1:2000, #2144, Cell Signaling Technology). ..

    Article Title: Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells.
    Article Snippet: Protein concentrations were determined using a BCA assay (Pierce), and equal amounts of protein from different samples were loaded onto a 4%–15% precast gel (Bio-Rad). .. SOX10 was detected using a mouse anti-SOX10 antibody (1:1,000, sc-271163, Santa Cruz Biotechnology). α-Tubulin was used as a protein loading control (1:2000, #2144, Cell Signaling Technology). ..

    Article Title: DPP an extracellular matrix molecule induces Wnt5a mediated signaling to promote the differentiation of adult stem cells into odontogenic lineage
    Article Snippet: .. Actin was used as protein loading control while tubulin and lamin A/C (#4777 Cell signaling) were used for cytoplasmic and nuclear fractions respectively. ..

    Article Title: MSC administration resolves experimental acute gout increasing specialized pro-resolving mediators synthesis through a super-induction of prostaglandin E 2 .
    Article Snippet: The following primary antibodies were applied overnight at 4°C: anti-human COX-2 1:1000 (Thermo Fisher Scientific); anti-human LOX-5 1:1000 (BD AR TIC LE IN PR ES S Biosciences); anti-rabbit LOX-12 1:500 (Abcam); anti-mouse LOX-15 1:500 (Abcam); and antihuman ARG-1 1:5000 (Proteintech). .. Protein loading control was performed employing Coomasie-blue staining for rabbit tissue samples [33], and β-actin 1:10000 (Cell Signaling) for isolated cell samples. ..

    Article Title: Involvement of lncRNAs NEAT1 and ZBTB11-AS1 in Active and Persistent HIV-1 Infection in C20 Human Microglial Cell Line
    Article Snippet: The membranes were analyzed with the Immobilion Forte Western HRP Substrate (Merck, Darmstadt, Germany) for 30 s to 2 min using the UVITEC Cambridge FireReader ® (UVITEC Cambridge, UK). .. GAPDH was evaluated as a protein loading control using a GAPDH mouse mAb (HRP-conjugated) antibody (1:1000 dilution) (Cell Signaling, #51332S). .. DNA was extracted from C20 cells obtained at 1, 4, and 21 days p.i. and their respective mocks using the Kit GeneJET Genomic DNA purification kit (ThermoFisher) according to the manufacturer’s instructions.

    Staining:

    Article Title: MSC administration resolves experimental acute gout increasing specialized pro-resolving mediators synthesis through a super-induction of prostaglandin E 2 .
    Article Snippet: The following primary antibodies were applied overnight at 4°C: anti-human COX-2 1:1000 (Thermo Fisher Scientific); anti-human LOX-5 1:1000 (BD AR TIC LE IN PR ES S Biosciences); anti-rabbit LOX-12 1:500 (Abcam); anti-mouse LOX-15 1:500 (Abcam); and antihuman ARG-1 1:5000 (Proteintech). .. Protein loading control was performed employing Coomasie-blue staining for rabbit tissue samples [33], and β-actin 1:10000 (Cell Signaling) for isolated cell samples. ..

    Isolation:

    Article Title: MSC administration resolves experimental acute gout increasing specialized pro-resolving mediators synthesis through a super-induction of prostaglandin E 2 .
    Article Snippet: The following primary antibodies were applied overnight at 4°C: anti-human COX-2 1:1000 (Thermo Fisher Scientific); anti-human LOX-5 1:1000 (BD AR TIC LE IN PR ES S Biosciences); anti-rabbit LOX-12 1:500 (Abcam); anti-mouse LOX-15 1:500 (Abcam); and antihuman ARG-1 1:5000 (Proteintech). .. Protein loading control was performed employing Coomasie-blue staining for rabbit tissue samples [33], and β-actin 1:10000 (Cell Signaling) for isolated cell samples. ..



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